Aquaporin-4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87679

Novus Biologicals, part of Bio-Techne
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NBP1-87679
NBP1-87679-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Human, Mouse, Rat, Porcine, Bovine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Block/Neutralize, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Aquaporin 4/AQP4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CPDVEFKRRFKEAFSKAAQQTKGSYMEVEDNRSQVETDDLILKPGVVHVIDVDRGEEKKGKDQSGEVLSSV

Reactivity Notes

Use in Rat reported in scientific literature (PMID:33634832). Bovine reactivity reported in scientific literature (PMID: 31233960).

Marker

Astrocytes Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aquaporin-4 Antibody

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679]

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679]

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Analysis in human cerebral cortex and liver tissues. Corresponding AQP4 RNA-seq data are presented for the same tissues.
Western Blot: Aquaporin-4 Antibody [NBP1-87679]

Western Blot: Aquaporin-4 Antibody [NBP1-87679]

Western Blot: Aquaporin-4 Antibody [NBP1-87679] - Analysis in human brain tissue.
Immunohistochemistry: Aquaporin-4 Antibody [NBP1-87679]

Immunohistochemistry: Aquaporin-4 Antibody [NBP1-87679]

Immunohistochemistry: Aquaporin-4 Antibody [NBP1-87679] - Staining of mouse cerebellum shows positivity in astrocytes, mainly in the molecular layer.
View 5 more images

Applications for Aquaporin-4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Frozen

Validated from verified customer review.

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Immunoprecipitation

Reported in scientific literature (PMID: 32843684)

Western Blot

0.04 - 0.4 ug/mL
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-87679 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2), 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aquaporin 4/AQP4

The Aquaporin-4 gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selectivechannels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Twoalternatively spliced tra

Long Name

Aquaporin 4

Alternate Names

AQP-4, AQP4, MIWC, WCH4

Entrez Gene IDs

361 (Human)

Gene Symbol

AQP4

UniProt

P55087 (Human)

Additional Aquaporin 4/AQP4 Products

Product Documents for Aquaporin-4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Aquaporin-4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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