Aquaporin-4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12902

Novus Biologicals, part of Bio-Techne
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NBP3-12902
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (95%), Human (92%), Porcine (92%), Rhesus Macaque (95%), Sheep (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Aquaporin 4/AQP4 Antibodies »

Product Summary for Aquaporin-4 Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse Aquaporin-4. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Aquaporin-4 Antibody

Western Blot: Aquaporin-4 Antibody [NBP3-12902]

Western Blot: Aquaporin-4 Antibody [NBP3-12902]

Western Blot: Aquaporin-4 Antibody [NBP3-12902] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Aquaporin-4 antibody (NBP3-12902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Aquaporin-4 Antibody [NBP3-12902]

Immunocytochemistry/ Immunofluorescence: Aquaporin-4 Antibody [NBP3-12902]

Immunocytochemistry/Immunofluorescence: Aquaporin-4 Antibody [NBP3-12902] - Aquaporin-4 antibody detects Aquaporin-4 protein at astrocyte cells by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aquaporin-4 protein stained by Aquaporin-4 antibody (NBP3-12902) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (NBP2-43561) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP3-12902]

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP3-12902]

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP3-12902] - Aquaporin-4 antibody detects Aquaporin-4 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Aquaporin-4 stained by Aquaporin-4 antibody (NBP3-12902) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for Aquaporin-4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry Free-Floating

Assay dependent

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aquaporin 4/AQP4

The Aquaporin-4 gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selectivechannels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Twoalternatively spliced tra

Long Name

Aquaporin 4

Alternate Names

AQP-4, AQP4, MIWC, WCH4

Gene Symbol

AQP4

Additional Aquaporin 4/AQP4 Products

Product Documents for Aquaporin-4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aquaporin-4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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