Aquaporin-5 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67247
![Western Blot: Aquaporin-5 AntibodyBSA Free [NBP2-67247]](https://resources.bio-techne.com/images/products/Aquaporin-5-Antibody-Western-Blot-NBP2-67247-img0001.jpg "Western Blot: Aquaporin-5 AntibodyBSA Free [NBP2-67247]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (90%), Porcine (90%), Rat (90%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for Aquaporin-5 Antibody - BSA Free
Immunogen
Synthetic peptide made to an N-terminal portion of the human Aquaporin-5 protein (between amino acids 170-250). [UniProt P55064]
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Aquaporin-5 Antibody - BSA Free
Western Blot: Aquaporin-5 AntibodyBSA Free [NBP2-67247]
Western Blot: Aquaporin-5 Antibody [NBP2-67247] - Total protein from human USO2, NTERA-2 and HeLa cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Aquaporin 5 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.![Immunocytochemistry/ Immunofluorescence: Aquaporin-5 Antibody - BSA Free [NBP2-67247]](https://resources.bio-techne.com/images/products/Aquaporin-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-67247-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Aquaporin-5 Antibody - BSA Free [NBP2-67247]")
Immunocytochemistry/ Immunofluorescence: Aquaporin-5 Antibody - BSA Free [NBP2-67247]
Immunocytochemistry/Immunofluorescence: Aquaporin-5 Antibody [NBP2-67247] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-AQP5 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.Applications for Aquaporin-5 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Western Blot
2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aquaporin 5/AQP5
Long Name
Aquaporin 5
Alternate Names
AQP-5, AQP5, PPKB
Gene Symbol
AQP5
Additional Aquaporin 5/AQP5 Products
Product Documents for Aquaporin-5 Antibody - BSA Free
Product Specific Notices for Aquaporin-5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...