Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1B3

Format

BSA Free

Concentration

1 mg/ml

View all ARF1 Antibodies »

Product Specifications

Immunogen

Recombinant human ARF1 (1-181aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ARF1 Antibody (1B3) - BSA Free

Western Blot: ARF1 Antibody (1B3)BSA Free [NBP1-97935]

Western Blot: ARF1 Antibody (1B3)BSA Free [NBP1-97935]

Western Blot: ARF1 Antibody (1B3) [NBP1-97935] - The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: Brain tissue lysate Lane 4.: MCF cell lysate
Immunocytochemistry/ Immunofluorescence: ARF1 Antibody (1B3) - BSA Free [NBP1-97935]

Immunocytochemistry/ Immunofluorescence: ARF1 Antibody (1B3) - BSA Free [NBP1-97935]

Immunocytochemistry/Immunofluorescence: ARF1 Antibody (1B3) [NBP1-97935] - Analysis in MCF7 cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ARF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ARF1 in U87MG cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ARF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: ARF1 Antibody (1B3) - BSA Free [NBP1-97935]

Flow Cytometry: ARF1 Antibody (1B3) - BSA Free [NBP1-97935]

Flow Cytometry: ARF1 Antibody (1B3) [NBP1-97935] - Analysis of ARF1 in MCF7 cell line, staining at2-5ug for 1x106cells (red line). The secondary antibody usedgoat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).

Applications for ARF1 Antibody (1B3) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARF1

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.

Long Name

ADP-ribosylation factor 1

Alternate Names

ADP-ribosylation factor 1

Gene Symbol

ARF1

UniProt

NP_ 001649 (Human)

Additional ARF1 Products

Product Documents for ARF1 Antibody (1B3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARF1 Antibody (1B3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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