ARF1 Antibody (ARF1/2117) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08473

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # ARF1/2117

Format

Azide and BSA Free

Concentration

1 mg/ml

View all ARF1 Antibodies »

Product Summary for ARF1 Antibody (ARF1/2117) - Azide and BSA Free

Immunogen

Recombinant full-length human ARF1 protein (Uniprot: P84077)

Localization

Cytoplasmic (Golgi Apparatus)

Marker

Golgi Apparatus Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07774).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ARF1 Antibody (ARF1/2117) - Azide and BSA Free

Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) - Azide and BSA Free [NBP3-08473]

Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) - Azide and BSA Free [NBP3-08473]

Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) - Azide and BSA Free [NBP3-08473] - Formalin-fixed, paraffin-embedded Rat Brain stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).
Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) - Azide and BSA Free [NBP3-08473]

Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) - Azide and BSA Free [NBP3-08473]

Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) - Azide and BSA Free [NBP3-08473] - Formalin-fixed, paraffin-embedded human Kidney stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).
Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) - Azide and BSA Free [NBP3-08473]

Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) - Azide and BSA Free [NBP3-08473]

Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) - Azide and BSA Free [NBP3-08473] - Formalin-fixed, paraffin-embedded human Liver stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).
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Applications for ARF1 Antibody (ARF1/2117) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: ARF1

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.

Long Name

ADP-ribosylation factor 1

Alternate Names

ADP-ribosylation factor 1

Gene Symbol

ARF1

Additional ARF1 Products

Product Documents for ARF1 Antibody (ARF1/2117) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARF1 Antibody (ARF1/2117) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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