ARF5 Antibody (0U0D2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16177

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16177-100ul
NBP3-16177-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0U0D2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ARF5 Antibodies »

Product Summary for ARF5 Antibody (0U0D2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 81-180 of human ARF5 (P84085). YFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARF5 Antibody (0U0D2)

Western Blot: ARF5 Antibody (0U0D2) [NBP3-16177]

Western Blot: ARF5 Antibody (0U0D2) [NBP3-16177]

Western Blot: ARF5 Antibody (0U0D2) [NBP3-16177] - Western blot analysis of extracts of various cell lines, using ARF5 Rabbit mAb (NBP3-16177) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: ARF5 Antibody (0U0D2) [NBP3-16177]

Immunocytochemistry/ Immunofluorescence: ARF5 Antibody (0U0D2) [NBP3-16177]

Immunocytochemistry/Immunofluorescence: ARF5 Antibody (0U0D2) [NBP3-16177] - Immunofluorescence analysis of NIH-3T3 cells using ARF5 Rabbit mAb (NBP3-16177) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for ARF5 Antibody (0U0D2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARF5

ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns.

Alternate Names

ADP-ribosylation factor 5

Gene Symbol

ARF5

Additional ARF5 Products

Product Documents for ARF5 Antibody (0U0D2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ARF5 Antibody (0U0D2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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