ARF5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31005

Novus Biologicals, part of Bio-Techne
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NBP1-31005
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Porcine (100%), Rat (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ARF5 Antibodies »

Product Summary for ARF5 Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ARF5. The exact sequence is proprietary.

Localization

Golgi apparatus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARF5 Antibody

Immunohistochemistry-Paraffin: ARF5 Antibody [NBP1-31005]

Immunohistochemistry-Paraffin: ARF5 Antibody [NBP1-31005]

Immunohistochemistry-Paraffin: ARF5 Antibody [NBP1-31005] - Paraffin-embedded human breast carcinoma. ARF5 antibody dilution: 1:500.
ARF5 Antibody

Western Blot: ARF5 Antibody [NBP1-31005] -

Western Blot: ARF5 Antibody [NBP1-31005] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ARF5 antibody (NBP1-31005) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ARF5 Antibody

Immunocytochemistry/ Immunofluorescence: ARF5 Antibody [NBP1-31005] -

Immunocytochemistry/ Immunofluorescence: ARF5 Antibody [NBP1-31005] - ARF5 antibody detects ARF5 protein at Golgi apparatus and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ARF5 stained by ARF5 antibody (NBP1-31005) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for ARF5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARF5

ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns.

Alternate Names

ADP-ribosylation factor 5

Entrez Gene IDs

381 (Human)

Gene Symbol

ARF5

UniProt

P84085 (Human)

Additional ARF5 Products

Product Documents for ARF5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ARF5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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