ARF6 Antibody (9E7T9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15391
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9E7T9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 76-175 of human ARF6 (P62330). HYYTGTQGLIFVVDCADRDRIDEARQELHRIINDREMRDAIILIFANKQDLPDAMKPHEIQEKLGLTRIRDRNWYVQPSCATSGDGLYEGLTWLTSNYKS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ARF6 Antibody (9E7T9)
Western Blot: ARF6 Antibody (9E7T9) [NBP3-15391]
Western Blot: ARF6 Antibody (9E7T9) [NBP3-15391] - Western blot analysis of extracts of various cell lines, using ARF6 Rabbit mAb (NBP3-15391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Immunocytochemistry/ Immunofluorescence: ARF6 Antibody (9E7T9) [NBP3-15391]
Immunocytochemistry/Immunofluorescence: ARF6 Antibody (9E7T9) [NBP3-15391] - Immunofluorescence analysis of C6 cells using ARF6 Rabbit mAb (NBP3-15391) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Western Blot: ARF6 Antibody (9E7T9) [NBP3-15391]
Western Blot: ARF6 Antibody (9E7T9) [NBP3-15391] - Western blot analysis of extracts of various cell lines, using ARF6 Rabbit mAb (NBP3-15391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.Applications for ARF6 Antibody (9E7T9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ARF6
Long Name
ADP-ribosylation factor 6
Alternate Names
EC 3.6.5.2
Gene Symbol
ARF6
Additional ARF6 Products
Product Documents for ARF6 Antibody (9E7T9)
Product Specific Notices for ARF6 Antibody (9E7T9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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