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Argininosuccinate Synthase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88868

Novus Biologicals, part of Bio-Techne
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NBP1-88868
NBP1-88868-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AKQHGIPIPVTPKNPWSMDENLMHISYEAGILENPKNQAPPGLYTKTQDPAKAPNTPDILEIEFKKGVPVKVTNVKDG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Argininosuccinate Synthase Antibody

Western Blot: Argininosuccinate Synthase Antibody [NBP1-88868]

Western Blot: Argininosuccinate Synthase Antibody [NBP1-88868]

Western Blot: Argininosuccinate Synthase Antibody [NBP1-88868] - ASS1 was successfully silenced in H4-II-E-C3 cells. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Argininosuccinate Synthase Antibody [NBP1-88868]

Immunocytochemistry/ Immunofluorescence: Argininosuccinate Synthase Antibody [NBP1-88868]

Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868]

Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868]

Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human skin shows moderate cytoplasmic positivity in a subset of squamous epithelial cells.

Applications for Argininosuccinate Synthase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP1-88868 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Argininosuccinate Synthase

Argininosuccinate Synthase is encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of Argininosuccinate Synthase cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Alternate Names

argininosuccinate synthase, argininosuccinate synthase 1, argininosuccinate synthetase, argininosuccinate synthetase 1, ASSCTLN1, citrulline-aspartate ligase, Citrulline--aspartate ligase, EC 6.3.4.5

Gene Symbol

ASS1

Additional Argininosuccinate Synthase Products

Product Documents for Argininosuccinate Synthase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Argininosuccinate Synthase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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