ARHGEF11 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06553

Novus Biologicals, part of Bio-Techne
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NBP1-06553
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all ARHGEF11 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1472 and 1522 of human PDZ-RhoGEF using the numbering given in entry NP_055599.1 (GeneID 9826).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARHGEF11 Antibody

Western Blot: ARHGEF11 Antibody [NBP1-06553]

Western Blot: ARHGEF11 Antibody [NBP1-06553]

Western Blot: ARHGEF11 Antibody [NBP1-06553] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-06553 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PDZ-RhoGEF was also immunoprecipitated by NBP1-06552, which recognizes an upstream epitope.
Immunohistochemistry-Paraffin: ARHGEF11 Antibody [NBP1-06553]

Immunohistochemistry-Paraffin: ARHGEF11 Antibody [NBP1-06553]

Immunohistochemistry-Paraffin: ARHGEF11 Antibody [NBP1-06553] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PDZ-RhoGEF used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for ARHGEF11 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in ICC/IF reported in scientific literature (PMID: 30219706).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ARHGEF11

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. A similar protein in rat interacts with glutamate transporter EAAT4 and modulates its glutamate transport activity. Expression of the rat protein induces the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq]

Alternate Names

DKFZp667F1223, glutamate transporter EAAT4-associated protein 48, GTRAP48, KIAA0380rho guanine nucleotide exchange factor 11, PDZ-RhoGEF, Rho guanine exchange factor (GEF) 11, Rho guanine nucleotide exchange factor (GEF) 11, RhoA-specific guanine nucleotide exchange factor, RhoGEF glutamate transport modulator

Entrez Gene IDs

9826 (Human)

Gene Symbol

ARHGEF11

UniProt

O15085 (Human)

Additional ARHGEF11 Products

Product Documents for ARHGEF11 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARHGEF11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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