ARHGEF5 Antibody (3A12-B5)
Novus Biologicals, part of Bio-Techne | Catalog # H00007984-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3A12-B5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ARHGEF5 (AAH14555, 1 a.a. ~ 519 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSETPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELIVSEASYLRSLNIAVDHFQLSTSLRATLSNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSSEEAEATKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTALEFSASPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLRQNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDGERGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQA
Specificity
ARHGEF5 - Rho guanine nucleotide exchange factor (GEF) 5
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for ARHGEF5 Antibody (3A12-B5)
![Western Blot: ARHGEF5 Antibody (3A12-B5) [H00007984-M01]](https://resources.bio-techne.com/images/products/ARHGEF5-Antibody-3A12-B5-Western-Blot-H00007984-M01-img0003.jpg "Western Blot: ARHGEF5 Antibody (3A12-B5) [H00007984-M01]")
Western Blot: ARHGEF5 Antibody (3A12-B5) [H00007984-M01]
Western Blot: ARHGEF5 Antibody (3A12-B5) [H00007984-M01] - Analysis of ARHGEF5 expression in transfected 293T cell line by ARHGEF5 monoclonal antibody (M01), clone 3A12-B5.Lane 1: ARHGEF5 transfected lysate(60.1 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: ARHGEF5 Antibody (3A12-B5) [H00007984-M01] - Detection limit for recombinant GST tagged ARHGEF5 is 1 ng/ml as a capture antibody.
Applications for ARHGEF5 Antibody (3A12-B5)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ARHGEF5
Alternate Names
ephexin-3, GEF5, Guanine nucleotide regulatory protein TIM, Oncogene TIM, P60, p60 TIM, Rho guanine nucleotide exchange factor (GEF) 5, rho guanine nucleotide exchange factor 5, TIM1, TIMDKFZp686N1969, Transforming immortalized mammary oncogene
Entrez Gene IDs
7984 (Human)
Gene Symbol
ARHGEF5
OMIM
600888 (Human)
UniProt
Additional ARHGEF5 Products
Product Documents for ARHGEF5 Antibody (3A12-B5)
Product Specific Notices for ARHGEF5 Antibody (3A12-B5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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