ARID1B Antibody (2F2)

Novus Biologicals, part of Bio-Techne | Catalog # H00057492-M02

Novus Biologicals, part of Bio-Techne
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H00057492-M02
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2F2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ARID1B Antibodies »

Product Specifications

Immunogen

ARID1B (NP_059989, 1364 a.a. ~ 1460 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPAKRHEGDMYNMQYSSQQQEMYNQYGGSYSGPDRRPIQGQYPYPYSRERMQGPGQIQTHGIPPQMMGGPLQSSSSEGPQQNMWAARNDMPYPYQNR

Specificity

ARID1B (2F2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for ARID1B Antibody (2F2)

Western Blot: ARID1B Antibody (2F2) [H00057492-M02]

Western Blot: ARID1B Antibody (2F2) [H00057492-M02]

Western Blot: ARID1B Antibody (2F2) [H00057492-M02] - ARID1B monoclonal antibody (M02), clone 2F2 Analysis of ARID1B expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: ARID1B Antibody (2F2) [H00057492-M02]

Immunocytochemistry/ Immunofluorescence: ARID1B Antibody (2F2) [H00057492-M02]

Immunocytochemistry/Immunofluorescence: ARID1B Antibody (2F2) [H00057492-M02] - Analysis of monoclonal antibody to ARID1B on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: ARID1B Antibody (2F2) [H00057492-M02]

Immunohistochemistry-Paraffin: ARID1B Antibody (2F2) [H00057492-M02]

Immunohistochemistry-Paraffin: ARID1B Antibody (2F2) [H00057492-M02] - Analysis of monoclonal antibody to ARID1B on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
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Applications for ARID1B Antibody (2F2)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using H00057492-M02 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARID1B

ARID1B is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration ofDNA-nucleosome topology). Binds DNA non-specifically

Alternate Names

6A3-5, ARID domain-containing protein 1B, AT rich interactive domain 1B (SWI1-like), BAF250b, BRG1-associated factor 250b, BRG1-binding protein ELD/OSA1, BRG1-binding protein hELD/OSA1, BRIGHT, DAN15P250R, ELD (eyelid)/OSA protein, ELD/OSA1, hOsa2, JPUT1, KDM5B, KIAA1235AT-rich interactive domain-containing protein 1B, Osa homolog 2, OSA2, p250R, PLU1, RBBP2H1A

Entrez Gene IDs

57492 (Human)

Gene Symbol

ARID1B

UniProt

NP_059989 (Human)

Additional ARID1B Products

Product Documents for ARID1B Antibody (2F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARID1B Antibody (2F2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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