ARID1B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35563
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 400-650 of human ARID1B (NP_059989.2).
Sequence:
GGFQRFAGQNQHPSGATPTLNQLLTSPSPMMRSYGGSYPEYSSPSAPPPPPSQPQSQAAAAGAAAGGQQAAAGMGLGKDMGAQYAAASPAWAAAQQRSHPAMSPGTPGPTMGRSQGSPMDPMVMKRPQLYGMGSNPHSQPQQSSPYPGGSYGPPGPQRYPIGIQGRTPGAMAGMQYPQQQMPPQYGQQGVSGYCQQGQQPYYSQQPQPPHLPPQAQYLPSQSQQRYQPQQDMSQEGYGTRSQPPLAPGKPN
Sequence:
GGFQRFAGQNQHPSGATPTLNQLLTSPSPMMRSYGGSYPEYSSPSAPPPPPSQPQSQAAAAGAAAGGQQAAAGMGLGKDMGAQYAAASPAWAAAQQRSHPAMSPGTPGPTMGRSQGSPMDPMVMKRPQLYGMGSNPHSQPQQSSPYPGGSYGPPGPQRYPIGIQGRTPGAMAGMQYPQQQMPPQYGQQGVSGYCQQGQQPYYSQQPQPPHLPPQAQYLPSQSQQRYQPQQDMSQEGYGTRSQPPLAPGKPN
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
244 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ARID1B Antibody
Western Blot: ARID1B Antibody [NBP3-35563] -
Western Blot: ARID1B Antibody [NBP3-35563] - Western blot analysis of various lysates using ARID1B Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry: ARID1B Antibody [NBP3-35563] -
Immunohistochemistry: ARID1B Antibody [NBP3-35563] - Immunohistochemistry analysis of paraffin-embedded Human breast using ARID1B Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunocytochemistry/ Immunofluorescence: ARID1B Antibody [NBP3-35563] -
Immunocytochemistry/ Immunofluorescence: ARID1B Antibody [NBP3-35563] - Immunofluorescence analysis of C6 cells using ARID1B Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Applications for ARID1B Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:200 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ARID1B
Alternate Names
6A3-5, ARID domain-containing protein 1B, AT rich interactive domain 1B (SWI1-like), BAF250b, BRG1-associated factor 250b, BRG1-binding protein ELD/OSA1, BRG1-binding protein hELD/OSA1, BRIGHT, DAN15P250R, ELD (eyelid)/OSA protein, ELD/OSA1, hOsa2, JPUT1, KDM5B, KIAA1235AT-rich interactive domain-containing protein 1B, Osa homolog 2, OSA2, p250R, PLU1, RBBP2H1A
Gene Symbol
ARID1B
Additional ARID1B Products
Product Documents for ARID1B Antibody
Product Specific Notices for ARID1B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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