ARID5B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13006
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human ARID5B. The exact sequence is proprietary.
Reactivity Notes
Rat (80%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
132 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for ARID5B Antibody
Mouse intestine. ARID5B stained by ARID5B antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Mouse intestine. ARID5B stained by ARID5B antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID5B antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID5B antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Mouse intestine. ARID5B stained by ARID5B antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Mouse intestine. ARID5B stained by ARID5B antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.Applications for ARID5B Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ARID5B
Alternate Names
ARID domain-containing protein 5B, AT rich interactive domain 5B (MRF1-like), DESRTAT-rich interactive domain-containing protein 5B, FLJ21150, Modulator recognition factor 2, modulator recognition factor 2 (MRF2), MRF-2, MRF2MRF1-like protein
Gene Symbol
ARID5B
Additional ARID5B Products
Product Specific Notices for ARID5B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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