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Aromatase Antibody (CYP19A1/4257) [Janelia Fluor® 635]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08711JF635

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Western Blot

Label

Janelia Fluor 635

Antibody Source

Monoclonal Mouse IgG2A Clone # CYP19A1/4257

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Aromatase Antibody (CYP19A1/4257) [Janelia Fluor® 635]

Immunogen

Synthetic peptide corresponding to aa376-390 of human Aromatase (KALEDDVIGYPVKK) (Uniprot: O14793)

Localization

Membrane

Specificity

CYP450 Aromatase is part of the CYP19A1 family involved in the aromatization of androgens to estrogens, a highly conserved mechanism amongst mammals. Clone CYP19A1/4257 recognizes a conserved epitope on the CYP450 aromatase, allowing for detection of aromatase levels using various analysis methods.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Aromatase Antibody (CYP19A1/4257) [Janelia Fluor® 635]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Aromatase

Aromatase is a member of the cytochrome P450 superfamily, whose function is to produce estrogens by aromatizing androgens. The primary function of Aromatase is to convert androstenedione to estrone and testosterone to estradiol. This protein is also a key enzyme in steroidogenesis, specifically for the biosynthesis of estrogens. Because estrogens also promote certain cancers and other diseases, aromatase inhibitors are frequently used to treat such diseases. It also plays an important role in sexual differentiation, fertility, and carcinogenesis.

Aromatase is localized in the endoplasmic reticulum of the cell and its activity is regulated by tissue specific promoters that are in turn controlled by hormones, cytokines, and other factors. It can be found in many tissues reproductive tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, endometrium as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer.

Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function. The activity is increased by environmental factors such as age, obesity, insulin, gonadotropins, and alcohol but decreased by prolactin, anti-m llerian hormone, and smoking.

Long Name

Aromatase

Alternate Names

ARO1, CYAR, CYP19, CYP19A1, CYPXIX, EC 1.14.14.14, Estrogen synthase

Gene Symbol

CYP19A1

Additional Aromatase Products

Product Documents for Aromatase Antibody (CYP19A1/4257) [Janelia Fluor® 635]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aromatase Antibody (CYP19A1/4257) [Janelia Fluor® 635]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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