ARP10 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91682
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CSSCAWELVDFIKAHDHLNLGIFASRLYYHWCKPQQKGLRLLCGSQVPVEVMGFPEFADCWENFVDHEKPLSFNPYKMLEELDKNSRAIKRRLERIKQS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ARP10 Antibody
Western Blot: ARP10 Antibody [NBP1-91682]
Western Blot: ARP10 Antibody [NBP1-91682] - Endogenous APOBEC3H stability/instability occurs at the protein level. Immunoblots showing endogenous A3H, A3G, and HSP90 protein levels in stimulated primary T lymphocytes from 4 donors with the indicated A3H haplotypes (donors 10, 11, 12, and 18). In this experiment endogenous A3H is detected with a polyclonal rabbit antibody. Image collected and cropped by CiteAb from the following publication (https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004761), licensed under a CC-BY license.Western Blot: ARP10 Antibody [NBP1-91682]
Western Blot: ARP10 Antibody [NBP1-91682] - A) APOBEC3H (A3H) haplotype II SV183 and various mutants expressed in 293T cells. B) The Novus anti-ARP10 antibody detected two splice variants of A3H, SV183 and SV200 in MCF-7 cell lysates. Transient transfection, overnight incubation with primary. WB image submitted by a verified customer review.Immunohistochemistry-Paraffin: ARP10 Antibody [NBP1-91682] -
Immunohistochemistry-Paraffin: ARP10 Antibody [NBP1-91682] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.Applications for ARP10 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reported in scientific literature (PMID 27650891)
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
Reported in scientific literature (PMID: 25411794)/verified customer review.
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval method is recommended.
Reviewed Applications
Read 1 review rated 5 using NBP1-91682 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ARP10
Alternate Names
APOBEC-related protein 10, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3H, ARP-10, ARP10Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3H, DNA dC->dU-editing enzyme APOBEC-3H, EC 3.5.4.-
Entrez Gene IDs
164668 (Human)
Gene Symbol
APOBEC3H
UniProt
Additional ARP10 Products
Product Documents for ARP10 Antibody
Product Specific Notices for ARP10 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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