Arylsulfatase B/ARSB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06021

Novus Biologicals, part of Bio-Techne
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NBP1-06021
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Arylsulfatase B/ARSB Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KLARGHTNGTKPLD corresponding to internal region according to NP_000037.2, NP_942002.1.

Epitope

C-KLARGHTNGTKPLD

Specificity

This antibody is expected to recognise both reported isoforms (NP_000037.2; NP_942002.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Arylsulfatase B/ARSB Antibody

Western Blot: Arylsulfatase B/ARSB Antibody [NBP1-06021]

Western Blot: Arylsulfatase B/ARSB Antibody [NBP1-06021]

Western Blot: Arylsulfatase B/ARSB Antibody [NBP1-06021] - (0.03ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Arylsulfatase B/ARSB Antibody [NBP1-06021]

Immunohistochemistry-Paraffin: Arylsulfatase B/ARSB Antibody [NBP1-06021]

Immunohistochemistry-Paraffin: Arylsulfatase B/ARSB Antibody [NBP1-06021] - Arylsulfatase B/ARSB Antibody [NBP1-06021] - (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Arylsulfatase B/ARSB Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 10 ug/ml

Immunohistochemistry-Paraffin

1:200

Peptide ELISA

detection limit 1:64000

Western Blot

0.03 ug/ml
Application Notes
WB: Approx. 45 kDa band observed in human heart lysates (calculated MW of 46.0 kDa band according to NP_942002.1). An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human skeletal muscle shows striated pattern staining of majority of muscular fibres.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Arylsulfatase B/ARSB

Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

ARSB, ASB, N-acetylgalactosamine-4-sulfatase

Entrez Gene IDs

411 (Human)

Gene Symbol

ARSB

UniProt

NP_000037.2 (Human)

Additional Arylsulfatase B/ARSB Products

Product Documents for Arylsulfatase B/ARSB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Arylsulfatase B/ARSB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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