Arylsulfatase B/ARSB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06021

Novus Biologicals, part of Bio-Techne
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NBP1-06021
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Arylsulfatase B/ARSB Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KLARGHTNGTKPLD corresponding to internal region according to NP_000037.2, NP_942002.1.

Epitope

C-KLARGHTNGTKPLD

Specificity

This antibody is expected to recognise both reported isoforms (NP_000037.2; NP_942002.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Arylsulfatase B/ARSB Antibody

Western Blot: Arylsulfatase B/ARSB Antibody [NBP1-06021]

Western Blot: Arylsulfatase B/ARSB Antibody [NBP1-06021]

Western Blot: Arylsulfatase B/ARSB Antibody [NBP1-06021] - (0.03ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Arylsulfatase B/ARSB Antibody [NBP1-06021]

Immunohistochemistry-Paraffin: Arylsulfatase B/ARSB Antibody [NBP1-06021]

Immunohistochemistry-Paraffin: Arylsulfatase B/ARSB Antibody [NBP1-06021] - Arylsulfatase B/ARSB Antibody [NBP1-06021] - (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Arylsulfatase B/ARSB Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 10 ug/ml

Immunohistochemistry-Paraffin

1:200

Peptide ELISA

detection limit 1:64000

Western Blot

0.03 ug/ml
Application Notes
WB: Approx. 45 kDa band observed in human heart lysates (calculated MW of 46.0 kDa band according to NP_942002.1). An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human skeletal muscle shows striated pattern staining of majority of muscular fibres.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Arylsulfatase B/ARSB

Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

ARSB, ASB, N-acetylgalactosamine-4-sulfatase

Entrez Gene IDs

411 (Human)

Gene Symbol

ARSB

UniProt

NP_000037.2 (Human)

Additional Arylsulfatase B/ARSB Products

Product Documents for Arylsulfatase B/ARSB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Arylsulfatase B/ARSB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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