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ASAH3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-74125

Novus Biologicals, part of Bio-Techne
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NBP1-74125

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptides corresponding to the C terminal of ACER1. Immunizing peptide sequence ITFPYGMVTMALVDANYEMPGETLKVRYWPRDSWPVGLPYVEIRGDDKDC. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for ASAH3 Antibody

Western Blot: ASAH3 Antibody [NBP1-74125]

Western Blot: ASAH3 Antibody [NBP1-74125]

Western Blot: ASAH3 Antibody [NBP1-74125] - Human Fetal Brain Lysate, concentration 1 ug/ml.

Applications for ASAH3 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASAH3

ACER1 hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0. It has a highly restricted substrate specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine but not D-ribo-phytosphingosine or D-erythro-dihydrosphingosine as a backbone. It may have a role in regulating the levels of bioactive lipids ceramide and sphingosine 1-phosphate, as well as complex sphingolipids.

Alternate Names

Acylsphingosine deacylase 3, Alkaline CDase 1, alkaline ceramidase 1, AlkCDase 1, ALKCDase1, ASAH3, EC 3.5.1.23, MGC138327, MGC138329, N-acylsphingosine amidohydrolase (alkaline ceramidase) 3, N-acylsphingosine amidohydrolase 3

Gene Symbol

ACER1

UniProt

Additional ASAH3 Products

Product Documents for ASAH3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ASAH3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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