ASCL1/Mash1 Antibody (7E11)

Novus Biologicals, part of Bio-Techne | Catalog # H00000429-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 7E11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ASCL1/Mash1 Antibodies »

Product Specifications

Immunogen

ASCL1 (NP_004307, 137 a.a. ~ 236 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GFATLREHVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSAAFQAGVLSPTISPNYSNDLNSMAGSPVSSYSSDEGSYDPLSPEEQELLDFTNWF

Specificity

ASCL1 - achaete-scute complex-like 1 (Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ASCL1/Mash1 Antibody (7E11)

Western Blot: ASCL1/Mash1 Antibody (7E11) [H00000429-M01]

Western Blot: ASCL1/Mash1 Antibody (7E11) [H00000429-M01]

Western Blot: ASCL1/Mash1 Antibody (7E11) [H00000429-M01] - Analysis of ASCL1 expression in transfected 293T cell line by ASCL1 monoclonal antibody (M01), clone 7E11.Lane 1: ASCL1 transfected lysate(25.5 KDa).Lane 2: Non-transfected lysate.
ELISA: ASCL1/Mash1 Antibody (7E11) [H00000429-M01]

ELISA: ASCL1/Mash1 Antibody (7E11) [H00000429-M01]

ELISA: ASCL1/Mash1 Antibody (7E11) [H00000429-M01] - ASCL1/Mash1 Antibody (7E11) [H00000429-M01] - Detection limit for recombinant GST tagged ASCL1 is approximately 1ng/ml as a capture antibody.

Applications for ASCL1/Mash1 Antibody (7E11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ASCL1/Mash1

This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.

Long Name

Achaete Scute Complex-like 1

Alternate Names

ASCL1, ASH1, HASH1, Mash1

Entrez Gene IDs

429 (Human)

Gene Symbol

ASCL1

OMIM

100790 (Human)

UniProt

NP_004307 (Human)

Additional ASCL1/Mash1 Products

Product Documents for ASCL1/Mash1 Antibody (7E11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ASCL1/Mash1 Antibody (7E11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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