ASCL1/Mash1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99418
![Western Blot: ASCL1/Mash1 Antibody [NBP2-99418]](https://resources.bio-techne.com/images/products/ASCL1-Mash1-Antibody-Western-Blot-NBP2-99418-img0006.jpg "Western Blot: ASCL1/Mash1 Antibody [NBP2-99418]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ASCL1/Mash1 Antibody
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ASCL1/Mash1
Specificity
Mouse MASH1/ASCL1
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ASCL1/Mash1 Antibody
Western Blot: ASCL1/Mash1 Antibody [NBP2-99418]
Western Blot: ASCL1/Mash1 Antibody [NBP2-99418] - Anti-ASCL1/Mash1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse brain tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 28 kDa![Immunoprecipitation: ASCL1/Mash1 Antibody [NBP2-99418]](https://resources.bio-techne.com/images/products/ASCL1-Mash1-Antibody-Immunoprecipitation-NBP2-99418-img0004.jpg "Immunoprecipitation: ASCL1/Mash1 Antibody [NBP2-99418]")
Immunoprecipitation: ASCL1/Mash1 Antibody [NBP2-99418]
Immunoprecipitation: ASCL1/Mash1 Antibody [NBP2-99418] - ASCL1/Mash1 was immunoprecipitated using: Lane A: 0.5 mg Mouse brains Tissue Lysate 4 ul anti-ASCL1/Mash1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-ASCL1/Mash1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 29 kDaApplications for ASCL1/Mash1 Antibody
Application
Recommended Usage
Immunoprecipitation
1-5 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ASCL1/Mash1
Long Name
Achaete Scute Complex-like 1
Alternate Names
ASCL1, ASH1, HASH1, Mash1
Gene Symbol
ASCL1
Additional ASCL1/Mash1 Products
Product Specific Notices for ASCL1/Mash1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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