ASCL1/Mash1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13159
Key Product Details
Species Reactivity
Validated:
Human, Rat
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse ASCL1/Mash1. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for ASCL1/Mash1 Antibody
![Immunocytochemistry/ Immunofluorescence: ASCL1/Mash1 Antibody [NBP3-13159]](https://resources.bio-techne.com/images/products/ASCL1-Mash1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13159-img0002.jpg "Immunocytochemistry/ Immunofluorescence: ASCL1/Mash1 Antibody [NBP3-13159]")
Immunocytochemistry/ Immunofluorescence: ASCL1/Mash1 Antibody [NBP3-13159]
Immunocytochemistry/Immunofluorescence: ASCL1/Mash1 Antibody [NBP3-13159] - ASCL1/Mash1 antibody detects ASCL1/Mash1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ASCL1/Mash1 stained by ASCL1/Mash1 antibody (NBP3-13159) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: ASCL1/Mash1 Antibody [NBP3-13159] -
Western Blot: ASCL1/Mash1 Antibody [NBP3-13159] - SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1/Mash1 antibody (NBP3-13159) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for ASCL1/Mash1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ASCL1/Mash1
Long Name
Achaete Scute Complex-like 1
Alternate Names
ASCL1, ASH1, HASH1, Mash1
Gene Symbol
ASCL1
Additional ASCL1/Mash1 Products
Product Specific Notices for ASCL1/Mash1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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