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ASF1b Antibody (004)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90279

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-90279-100ul
NBP2-90279-50ul

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 004

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human ASF1b (Uniprot#: Q9NVP2; Met1-Ile202).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ASF1b Antibody (004)

Western Blot: ASF1b Antibody (004) [NBP2-90279]

Western Blot: ASF1b Antibody (004) [NBP2-90279]

Western Blot: ASF1b Antibody (004) [NBP2-90279] - Lane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa
Immunoprecipitation: ASF1b Antibody (004) [NBP2-90279]

Immunoprecipitation: ASF1b Antibody (004) [NBP2-90279]

Immunoprecipitation: ASF1b Antibody (004) [NBP2-90279] - Lane A:0.5 mg K562 Whole Cell Lysate0.5 uL anti-ASF1B rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-ASF1B rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa

Applications for ASF1b Antibody (004)

Application
Recommended Usage

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASF1b

The ASF1b gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencingfunction-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cellcycle-regulated kinases, and may play

Alternate Names

ASF1 silencing function 1 homolog B (S. cerevisiae), CCG1-interacting factor A-II, CIA-II, FLJ10604, hAsf1, hAsf1b, hCIA-II, histone chaperone ASF1B, silencing function protein 1 homolog B

Gene Symbol

ASF1B

Additional ASF1b Products

Product Documents for ASF1b Antibody (004)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ASF1b Antibody (004)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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