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ASGPR1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15489

Novus Biologicals, part of Bio-Techne
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NBP2-15489

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for ASGPR1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ASGPR1. The exact sequence is proprietary.

Reactivity Notes

Rat (80%), Pig (84%), Bovine (82%).

Localization

Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ASGPR1 Antibody

Western Blot: ASGPR1 Antibody [NBP2-15489]

Western Blot: ASGPR1 Antibody [NBP2-15489]

Western Blot: ASGPR1 Antibody [NBP2-15489] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ASGPR1 antibody [N1C3] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
ASGPR1 Antibody

Western Blot: ASGPR1 Antibody [NBP2-15489] -

Western Blot: ASGPR1 Antibody [NBP2-15489] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ASGPR1 antibody [N1C3] (NBP2-15489) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ASGPR1 Antibody

Immunocytochemistry/ Immunofluorescence: ASGPR1 Antibody [NBP2-15489] -

Immunocytochemistry/ Immunofluorescence: ASGPR1 Antibody [NBP2-15489] - ASGPR1 antibody [N1C3] detects ASGPR1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASGPR1 stained by ASGPR1 antibody [N1C3] (NBP2-15489) diluted at 1:500.
Blue: Fluoroshield with DAPI .
Scale bar= 10um.

Applications for ASGPR1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ASGR1/ASGPR1

Partially deglycosylated plasma glycoproteins and immunoglobulin IgA2 allotypes are efficiently and specifically removed from circulation by a receptor-mediated process. The asialoglycoprotein receptor binds to desialylated (galactosyl-terminal) glycoproteins. It transports these glycoproteins via a series of membrane vesicles and tubules to an acidic-sorting organelle where the receptor and ligand dissociate. Then the receptor is recycled back to the cell surface and the ligand is transported to the lysosomes for degradation. [provided by RefSeq]

Long Name

Asialoglycoprotein Receptor 1

Alternate Names

ASGPR1, CLEC4H1, MHL1, RHL1

Gene Symbol

ASGR1

UniProt

Additional ASGR1/ASGPR1 Products

Product Documents for ASGPR1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ASGPR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.