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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 2919 and 2969 of human absent, small and homeotic disks protein 1 using the numbering given in entry CAI12722.1 (GeneID 55870).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASH1 Antibody - BSA Free

Western Blot: ASH1 Antibody [NB100-93290]

Western Blot: ASH1 Antibody [NB100-93290]

Western Blot: ASH1 Antibody [NB100-93290] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds
Immunohistochemistry-Paraffin: ASH1 Antibody [NB100-93290]

Immunohistochemistry-Paraffin: ASH1 Antibody [NB100-93290]

Immunohistochemistry-Paraffin: ASH1 Antibody [NB100-93290] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: ASH1 Antibody [NB100-93290]

Western Blot: ASH1 Antibody [NB100-93290]

Western Blot: ASH1 Antibody [NB100-93290] - Detection of Human ASH1 on HeLa whole cell lysate using NB100-93290. ASH1 was also immunoprecipitated by rabbit anti-ASH1 antibody NB100-93289.

Applications for ASH1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ASH1

This gene encodes a member of the trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions. [provided by RefSeq]

Alternate Names

Absent small and homeotic disks protein 1 homolog, ASH1, ash1 (absent, small, or homeotic)-like (Drosophila), ASH1L1, ASH1-like protein, EC 2.1.1.43, FLJ10504, huASH1, KMT2HKIAA1420, Lysine N-methyltransferase 2H, probable histone-lysine N-methyltransferase ASH1L

Entrez Gene IDs

55870 (Human)

Gene Symbol

ASH1L

Additional ASH1 Products

Product Documents for ASH1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ASH1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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