Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ASH1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 2919 and 2969 of human absent, small and homeotic disks protein 1 using the numbering given in entry CAI12722.1 (GeneID 55870).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASH1 Antibody

Western Blot: ASH1 Antibody [NB100-93290]

Western Blot: ASH1 Antibody [NB100-93290]

Western Blot: ASH1 Antibody [NB100-93290] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds
Immunohistochemistry-Paraffin: ASH1 Antibody [NB100-93290]

Immunohistochemistry-Paraffin: ASH1 Antibody [NB100-93290]

Immunohistochemistry-Paraffin: ASH1 Antibody [NB100-93290] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: ASH1 Antibody [NB100-93290]

Western Blot: ASH1 Antibody [NB100-93290]

Western Blot: ASH1 Antibody [NB100-93290] - Detection of Human ASH1 on HeLa whole cell lysate using NB100-93290. ASH1 was also immunoprecipitated by rabbit anti-ASH1 antibody NB100-93289.
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Applications for ASH1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ASH1

This gene encodes a member of the trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions. [provided by RefSeq]

Alternate Names

Absent small and homeotic disks protein 1 homolog, ASH1, ash1 (absent, small, or homeotic)-like (Drosophila), ASH1L1, ASH1-like protein, EC 2.1.1.43, FLJ10504, huASH1, KMT2HKIAA1420, Lysine N-methyltransferase 2H, probable histone-lysine N-methyltransferase ASH1L

Entrez Gene IDs

55870 (Human)

Gene Symbol

ASH1L

UniProt

CAI12722.1 (Human)

Additional ASH1 Products

Product Documents for ASH1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ASH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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