ASH2L Antibody (10B6U5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16505

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16505-100ul
NBP3-16505-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10B6U5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ASH2L Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 529-628 of human ASH2L (Q9UBL3). AEKSLKQTPHSEIIFYKNGVNQGVAYKDIFEGVYFPAISLYKSCTVSINFGPCFKYPPKDLTYRPMSDMGWGAVVEHTLADVLYHVETEVDGRRSPPWEP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASH2L Antibody (10B6U5)

Western Blot: ASH2L Antibody (10B6U5) [NBP3-16505]

Western Blot: ASH2L Antibody (10B6U5) [NBP3-16505]

Western Blot: ASH2L Antibody (10B6U5) [NBP3-16505] - Analysis of extracts of various cell lines, using ASH2L Rabbit mAb (NBP3-16505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunocytochemistry/ Immunofluorescence: ASH2L Antibody (10B6U5) [NBP3-16505]

Immunocytochemistry/ Immunofluorescence: ASH2L Antibody (10B6U5) [NBP3-16505]

Immunocytochemistry/Immunofluorescence: ASH2L Antibody (10B6U5) [NBP3-16505] - Immunofluorescence analysis of U-2 OS cells using ASH2L Rabbit mAb (NBP3-16505) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: ASH2L Antibody (10B6U5) [NBP3-16505]

Immunohistochemistry-Paraffin: ASH2L Antibody (10B6U5) [NBP3-16505]

Immunohistochemistry-Paraffin: ASH2L Antibody (10B6U5) [NBP3-16505] - Mouse brain using ASH2L Rabbit mAb (NBP3-16505) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for ASH2L Antibody (10B6U5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ASH2L

ASH2L is a component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. May function as a transcriptional regulator. May play a rol

Alternate Names

ASH2, ash2 (absent, small, or homeotic)-like (Drosophila), ASH2L1ash2 (absent, small, or homeotic, Drosophila, homolog)-like, ASH2L2ASH2-like protein, Bre2, set1/Ash2 histone methyltransferase complex subunit ASH2

Gene Symbol

ASH2L

Additional ASH2L Products

Product Documents for ASH2L Antibody (10B6U5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ASH2L Antibody (10B6U5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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