ASH2L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88858

Novus Biologicals, part of Bio-Techne
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NBP1-88858
NBP1-88858-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ASH2L Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TTGTTKKARSDPLFSAQRLPPHGYPLEHPFNKDGYRYILAEPDPHAPDPEKLELDCWAGKPIPGDLYRACLYERVLLALHDRAPQLKISDDRLTVVGEKGYSMVRASHGVRKGAWYFEITVDEM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASH2L Antibody

Western Blot: ASH2L Antibody [NBP1-88858]

Western Blot: ASH2L Antibody [NBP1-88858]

Western Blot: ASH2L Antibody [NBP1-88858] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: ASH2L Antibody [NBP1-88858]

Immunocytochemistry/ Immunofluorescence: ASH2L Antibody [NBP1-88858]

Immunocytochemistry/Immunofluorescence: ASH2L Antibody [NBP1-88858] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ASH2L Antibody [NBP1-88858]

Immunohistochemistry-Paraffin: ASH2L Antibody [NBP1-88858]

Immunohistochemistry-Paraffin: ASH2L Antibody [NBP1-88858] - Staining of human tonsil shows nuclear positivity in non-germinal center.
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Applications for ASH2L Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASH2L

ASH2L is a component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. May function as a transcriptional regulator. May play a rol

Alternate Names

ASH2, ash2 (absent, small, or homeotic)-like (Drosophila), ASH2L1ash2 (absent, small, or homeotic, Drosophila, homolog)-like, ASH2L2ASH2-like protein, Bre2, set1/Ash2 histone methyltransferase complex subunit ASH2

Gene Symbol

ASH2L

Additional ASH2L Products

Product Documents for ASH2L Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ASH2L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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