ASK1 Antibody (2E4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37592

Novus Biologicals, part of Bio-Techne
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NBP2-37592
NBP2-37592-0.025ml
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2E4

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all ASK1 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human ASK1 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ASK1 Antibody (2E4) - BSA Free

Western Blot: ASK1 Antibody (2E4)BSA Free [NBP2-37592]

Western Blot: ASK1 Antibody (2E4)BSA Free [NBP2-37592]

Western Blot: ASK1 Antibody (2E4) [NBP2-37592] - Western blot analysis using MAP3K5 mAb against human MAP3K5 (AA: 922-1108) recombinant protein. (Expected MW is 46.5 kDa)
Immunohistochemistry: ASK1 Antibody (2E4) - BSA Free [NBP2-37592]

Immunohistochemistry: ASK1 Antibody (2E4) - BSA Free [NBP2-37592]

Immunohistochemistry: ASK1 Antibody (2E4) [NBP2-37592] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and prostate tissues (right) using MAP3K5 mouse mAb with DAB staining.
Flow Cytometry: ASK1 Antibody (2E4) - BSA Free [NBP2-37592]

Flow Cytometry: ASK1 Antibody (2E4) - BSA Free [NBP2-37592]

Flow Cytometry: ASK1 Antibody (2E4) [NBP2-37592] - Flow cytometric analysis of Hela cells using MAP3K5 mouse mAb (green) and negative control (purple).
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Applications for ASK1 Antibody (2E4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASK1

Apoptosis signal-regulating kinase-1 (ASK1, MEKK5, MAPKKK5) is a MAP3k type protein kinase belonging to mitogen-activated protein kinase kinase kinase (MAPKKK) family. As a regulator of JNK and p38 MAPK signaling pathways, ASK1/MEKK5 phosphorylates MMK4 to activate c-Jun N-terminal kinase (JNK) and phosphorylates MEK3 and MKK6 to activate p38. ASK1/MEKK5 is activated by oxidative stress, TNF via TRAF2, Fas via Daxx, calcium overload, and endoplasmic reticulum stress (1). ASK1/MEKK5 is inactivated by Trx, Grx, 14-3-3, and protein serine/threonine phosphatase 5 (2). While elevated level of ASK1/MEKK5 can induce apoptosis, a catalytically inactive ASK1/MEKK5 acts as an inhibitor in TNF-alpha induced apoptosis. Over-expression of ASK1/MEKK5 has also been implicated in cell differentiation, such as in keratinocyte differentiation through p38 pathway (3). ASK1/MEKK5 has been implicated as a therapeutic target for neurodegenerative disorder sand cardiac dysfunction (4).

Long Name

Apoptosis Signal-regulated Kinase 1

Alternate Names

MAP3K5, MEKK5

Gene Symbol

MAP3K5

Additional ASK1 Products

Product Documents for ASK1 Antibody (2E4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ASK1 Antibody (2E4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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