ASK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56077

Novus Biologicals, part of Bio-Techne
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NB100-56077
NB100-56077SS
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all ASK1 Antibodies »

Product Summary for ASK1 Antibody

Immunogen

A synthetic peptide corresponding to amino acids 1198 to 1216(CHEEQPSNQTVRRPQAVIED) of human Ask1 was used as immunogen; GenBank no. NP_005914.1. Human Ask1 is a 1374 amino acid protein. The peptide immunogen sequence is 100% conserved in Ask1 variant (BAD92330.1), a 1455 amino acid protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASK1 Antibody

Immunohistochemistry-Paraffin: ASK1 Antibody [NB100-56077]

Immunohistochemistry-Paraffin: ASK1 Antibody [NB100-56077]

Immunohistochemistry-Paraffin: ASK1 Antibody [NB100-56077] - Formalin-fixed paraffin-embedded tissue section of human small venous vessels stained for ASK1 expression using this antibody at 1:2000. ASK1 positive epithelial cells were seen. Hematoxylin-eosin counterstain.
Immunohistochemistry-Paraffin: ASK1 Antibody [NB100-56077]

Immunohistochemistry-Paraffin: ASK1 Antibody [NB100-56077]

Immunohistochemistry-Paraffin: ASK1 Antibody [NB100-56077] - Formalin-fixed paraffin-embedded tissue section of human reactive tonsil stained for ASK1 expression using NB100-56077 at 1:2000. A and A1. Low and high magnification, respectively. ASK1 expression was seen in the vasculature (endothelial cells). Hematoxylin-eosin counterstain.

Applications for ASK1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASK1

Ask1 (apoptosis signal-regulating kinase 1), a serine/threonine kinase, is a member of the MAPKKK family that constitutes the JNK and p38 (MAPK) cascades. Members of this family are activated by physiological and cytotoxic stresses and induce various stress responses including apoptosis (reviewed in Sekine et al. 2006). Specifically, Ask1-MAPK cascades have been shown to be involved in endoplasmic reticululum (ER) stress-induced apoptosis and in neuronal cell death. Studies suggest that ER stress-induced apoptosis plays critical roles in the pathogenesis in some neurodegenerative diseases including Alzheimer's, Parkinson's, polyglutamine (polyQ), amyotrophic lateral sclerosis (ALS) and Prion diseases. Studies using using cultured mammalian cells suggest that Ask1 may play a role as a proapoptotic signaling intermediate in the pathogenesis of these and other neurodegenerative diseases that involve ER stress-induced apoptosis. However, the regulation and function of Ask1 in neuronal cell death remains to be fully elucidated. In addition to playing a role in ER stress-induced apoptosis, Ask1 is also thought to be important in cytokine-induced apoptosis, and may also have functions other than apoptosis including roles in cell survival and differentiation. Structurally, human Ask1 has a serine/threonine kinase domain in the middle of the molecule. The kinase domain is evolutionarily conserved from nematode to human. A threonine residue with the activation loop of the kinases domain (Thr838 of human Ask1) is essential for Ask1 activation. There are two coiled-coil domains, one in the N-terminal and the other in the C-terminal domain. The coiled-coil domain in the C-terminal domain has been shown to be required for homo-oligomerzation.

Long Name

Apoptosis Signal-regulated Kinase 1

Alternate Names

MAP3K5, MEKK5

Entrez Gene IDs

4217 (Human)

Gene Symbol

MAP3K5

Additional ASK1 Products

Product Documents for ASK1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ASK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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