ASK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81788

Novus Biologicals, part of Bio-Techne
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NB100-81788-0.025ml
NB100-81788
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ASK1 Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human ASK1 around amino acids 964~968 (S-I-S-L-P).

Specificity

Detects endogenous levels of total ASK1 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

155 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ASK1 Antibody

Western Blot: ASK1 Antibody [NB100-81788] -

Western Blot: ASK1 Antibody [NB100-81788] - Analysis of extracts from 293 and Hela cells using NB100-81788

Immunocytochemistry/Immunofluorescence: ASK1 Antibody [NB100-81788] -

Immunocytochemistry/Immunofluorescence: ASK1 Antibody [NB100-81788] - Staining of methanol-fixed Hela cells using NB100-81788

Immunohistochemistry-Paraffin: ASK1 Antibody [NB100-81788] -

Immunohistochemistry-Paraffin: ASK1 Antibody [NB100-81788] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81788 (left) or the same antibody preincubated with blocking peptide(right).

Applications for ASK1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASK1

Mitogen activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1). ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activate c-Jun N terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas associated protein Daxx. Over expression of ASK1 induces apoptotic cell death, and a catalytically inactive form of ASK1 inhibits TNF alpha-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.

Long Name

Apoptosis Signal-regulated Kinase 1

Alternate Names

MAP3K5, MEKK5

Entrez Gene IDs

4217 (Human); 26408 (Mouse)

Gene Symbol

MAP3K5

UniProt

Q99683

Additional ASK1 Products

Product Documents for ASK1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ASK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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