ASPM Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2278
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Porcine, Bovine, Ovine
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.25 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 3425 and the C-terminus (residue 3477) of human ASP (Abnormal Spindle)-like, Microcephaly Associated using the numbering given in entry NP_060606.2 (GeneID 259266).
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 22350525).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ASPM Antibody
Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278]
Immunocytochemistry/Immunofluorescence: ASPM Antibody [NB100-2278] - Samples: NBF-fixed asynchronous HeLa cells . Antibody: Affinity purified rabbit anti-ASPM used at a dilution of 1:250. Detection: Goat anti-rabbit IgG (h&l) FITC used at a dilution of 1:100.Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278]
Immunocytochemistry/Immunofluorescence: ASPM Antibody [NB100-2278] - Detection of Human ASPM in NBF-fixed asynchronous HeLa cells with anti-ASPM used at a dilution of 1:250. Detection: Goat anti-rabbit IgG (h&l) FITC used at a dilution of 1:100.Applications for ASPM Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 - 1:500
Immunohistochemistry
1:100 - 1:500
Application Notes
For ICC/IF: Permeabilization with Triton-X 100 is recommended. Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.Likely to work with frozen sections. IHC was reported in scientific literature (PMID: 22350525). Use in IHC-P reported in scientific literature (PMID: 30092011).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.25 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ASPM
Alternate Names
ASP, asp (abnormal spindle) homolog, microcephaly associated (Drosophila), Asp homolog, Calmbp1, FLJ43117, MCPH5DKFZp686N06184, microcephaly associated (Drosophila), microcephaly, primary autosomal recessive 5
Entrez Gene IDs
259266 (Human)
Gene Symbol
ASPM
UniProt
Additional ASPM Products
Product Documents for ASPM Antibody
Product Specific Notices for ASPM Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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