Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 103C411.2

Concentration

0.5 mg/ml

View all ATF2 Antibodies »

Product Specifications

Immunogen

This antibody was generated by immunizing mice with a synthetic peptide containing phosphorylated threonine at position 71 of human ATF-2.

Modification

p Thr71

Specificity

This monoclonal antibody is specific for phospho (Thr71) ATF2. It cannot be used to test for non-phosphorylated ATF2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ATF2 [p Thr71] Antibody (103C411.2)

Western Blot: ATF2 [p Thr71] Antibody (103C411.2) [NB100-56594] - Detection of ATF2 phosphorylation in HeLa cell lysate with phospho ATF2 antibody. Lane A. Untreated cell lysate; Lane B. HeLa cells treated with TNF for 1 hr.

Applications for ATF2 [p Thr71] Antibody (103C411.2)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Western Blot

2 ug/ml

Reviewed Applications

Read 1 review rated 5 using NB100-56594 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATF2

ATF-2 is a member of the group of bZip transcription factors. Heterodimer formation between members of the bZip group is common and is believed to add diversity to the cis-acting elements at which binding of the dimers is directed. Specifically, ATF-2 may dimerize with c-Jun, as occurs in response to E1a, and in so doing shift the binding preference of c-Jun toward ATF/CRE sites. Deletion analysis has indicated that the N-terminal region of ATF-2 containing threonine at residues 69 and 71 are essential for this purpose. These threonine residues are phosphorylated by JNK/SAPK for transcriptional activation.

Long Name

Activating Transcription Factor 2

Alternate Names

CRE-BP1, CREB2, HB16, mXBP, TREB7

Entrez Gene IDs

1386 (Human)

Gene Symbol

ATF2

UniProt

P15336

Additional ATF2 Products

Product Documents for ATF2 [p Thr71] Antibody (103C411.2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATF2 [p Thr71] Antibody (103C411.2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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