ATF3 Antibody (CL1685)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34489

Novus Biologicals, part of Bio-Techne
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NBP2-34489
NBP2-34489-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Rat, Feline

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry Free-Floating

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1685

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATF3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MMLQHPGQVSASEVSASAIVPCLSPPGSLVFEDFANLTPFVKEELRFAIQNKHLCHRMSSALESVTVSDRPLGVSITKAEVAPEEDERKKRRRERNKIAAAKCRNKKKEKTEC

Epitope

ASAIVPCLSPPGSLV

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ATF3 Antibody (CL1685)

Immunohistochemistry-Paraffin: ATF3 Antibody (CL1685) [NBP2-34489]

Immunohistochemistry-Paraffin: ATF3 Antibody (CL1685) [NBP2-34489]

Immunohistochemistry-Paraffin: ATF3 Antibody (CL1685) [NBP2-34489] - Staining in human placenta and tonsil tissues. Corresponding ATF3 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: ATF3 Antibody (CL1685) [NBP2-34489]

Immunocytochemistry/ Immunofluorescence: ATF3 Antibody (CL1685) [NBP2-34489]

Immunocytochemistry/Immunofluorescence: ATF3 Antibody (CL1685) [NBP2-34489] - Staining of RH-30 cells using the Anti-ATF3 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ATF3 Antibody (CL1685) [NBP2-34489]

Immunohistochemistry-Paraffin: ATF3 Antibody (CL1685) [NBP2-34489]

Immunohistochemistry-Paraffin: ATF3 Antibody (CL1685) [NBP2-34489] - Staining of human stomach cancer shows moderate to strong nuclear positivity in tumor cells.
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Applications for ATF3 Antibody (CL1685)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATF3

Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.

Alternate Names

activating transcription factor 3cAMP-dependent transcription factor ATF-3, ATF3deltaZip2, ATF3deltaZip2c, ATF3deltaZip3, cyclic AMP-dependent transcription factor ATF-3

Gene Symbol

ATF3

UniProt

P18847 (Human)

Additional ATF3 Products

Product Documents for ATF3 Antibody (CL1685)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATF3 Antibody (CL1685)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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