ATF5 Antibody (9D8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26496
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9D8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human ATF5 [UniProt Q9Y2D1]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ATF5 Antibody (9D8)
Immunohistochemistry: ATF5 Antibody (9D8) [NBP3-26496] -
Immunohistochemistry: ATF5 Antibody (9D8) [NBP3-26496] - Image of ATF5 Antibody (9D8) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: ATF5 Antibody (9D8) [NBP3-26496] -
Western Blot: ATF5 Antibody (9D8) [NBP3-26496] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, Rat brain tissue.All lanes: ATF5 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 31 kDa
Observed band size: 35 kDa
Applications for ATF5 Antibody (9D8)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: ATF5
Alternate Names
activating transcription factor 5FLJ34666, ATFXHMFN0395, cAMP-dependent transcription factor ATF-5, cyclic AMP-dependent transcription factor ATF-5, Transcription factor ATFx
Gene Symbol
ATF5
Additional ATF5 Products
Product Documents for ATF5 Antibody (9D8)
Product Specific Notices for ATF5 Antibody (9D8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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