ATF5 Antibody (9Z3M0)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16221
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9Z3M0
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 183-282 of human ATF5 (Q9Y2D1). PQQPPPPSPPQPSRLAPYPHPATTRGDRKQKKRDQNKSAALRYRQRKRAEGEALEGECQGLEARNRELKERAESVEREIQYVKDLLIEVYKARSQRTRSC
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ATF5 Antibody (9Z3M0)
![Western Blot: ATF5 Antibody (9Z3M0) [NBP3-16221]](https://resources.bio-techne.com/images/products/ATF5-Antibody-9Z3M0-Western-Blot-NBP3-16221-img0002.jpg "Western Blot: ATF5 Antibody (9Z3M0) [NBP3-16221]")
Western Blot: ATF5 Antibody (9Z3M0) [NBP3-16221]
Western Blot: ATF5 Antibody (9Z3M0) [NBP3-16221] - Western blot analysis of extracts of various cell lines, using ATF5 Rabbit mAb (NBP3-16221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.![Immunohistochemistry-Paraffin: ATF5 Antibody (9Z3M0) [NBP3-16221]](https://resources.bio-techne.com/images/products/ATF5-Antibody-9Z3M0-Immunohistochemistry-Paraffin-NBP3-16221-img0001.jpg "Immunohistochemistry-Paraffin: ATF5 Antibody (9Z3M0) [NBP3-16221]")
Immunohistochemistry-Paraffin: ATF5 Antibody (9Z3M0) [NBP3-16221]
Immunohistochemistry-Paraffin: ATF5 Antibody (9Z3M0) [NBP3-16221] - Immunohistochemistry of paraffin-embedded human placenta using ATF5 Rabbit mAb (NBP3-16221) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for ATF5 Antibody (9Z3M0)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ATF5
Alternate Names
activating transcription factor 5FLJ34666, ATFXHMFN0395, cAMP-dependent transcription factor ATF-5, cyclic AMP-dependent transcription factor ATF-5, Transcription factor ATFx
Gene Symbol
ATF5
Additional ATF5 Products
Product Documents for ATF5 Antibody (9Z3M0)
Product Specific Notices for ATF5 Antibody (9Z3M0)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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