ATG10 Antibody

Knockdown Validated: ATG10 Antibody [NBP2-38524] -

Knockdown Validated: ATG10 Antibody [NBP2-38524] - ARPE-19 cells were transfected with pooled ATG10 specific siRNAs or scrambled siRNA (30nM) for 24h. ATG10 and ATG5 levels were assessed in protein lysates by Western Blot. (B) RNA isolated from cells was analyzed for ATG10 mRNA expression by qRT- PCR. Differences between means were considered statistically significant when p<0.05 by Mann-Whitney U-test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28222108/), licensed under a CC-BY license.

Western Blot: ATG10 Antibody [NBP2-38524] -

Western Blot: ATG10 Antibody [NBP2-38524] - Knockdown of ATG10 makes ARPE-19 cells more sensitive to H2O2.(A) ARPE-19 cells were transfected with pooled ATG10 specific siRNAs or scrambled siRNA (30nM) for 24h. ATG10 & ATG5 levels were assessed in protein lysates by Western Blot. (B) RNA isolated from cells was analyzed for ATG10 mRNA expression by qRT- PCR. Differences between means were considered statistically significant when p<0.05 by Mann-Whitney U-test. (C) Lysates of ARPE-19 cells transfected with siRNAs for 24 hours were assessed for p62 & LC3 levels by Western blot. One set of wells per experiment was exposed to Bafilomycin A1 (75nM) for 3h prior to harvest. beta-actin was used as an internal control. ‘Short’ & ‘Long’ Exposure indicate time of exposure of immunoblot image. (D) 24h after transfection with respective siRNAs, ARPE-19 were subjected to oxidative stress with 800μM H2O2 & incubated at 37°C for 6h. Total cell numbers were determined by trypsinization & counting on TC10® cell counter. (E) Primary fetal RPE cells were transfected with scrambled or pooled ATG10 specific siRNAs & 48 hours after transfection, were subjected to H2O2 for 24 hours & MTT assay was performed. Differences between means were considered statistically significant when p<0.05 by Student’s t-test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28222108), licensed under a CC-BY license. Not internally tested by Novus Biologicals.