ATG12 Antibody (9R8W2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15812

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15812-100ul
NBP3-15812-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9R8W2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATG12 Antibodies »

Product Summary for ATG12 Antibody (9R8W2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 41-140 of human Apg12 (O94817). SPGTEEPAGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATG12 Antibody (9R8W2)

Western Blot: ATG12 Antibody (9R8W2) [NBP3-15812]

Western Blot: ATG12 Antibody (9R8W2) [NBP3-15812]

Western Blot: ATG12 Antibody (9R8W2) [NBP3-15812] - Western blot analysis of extracts of various cell lines, using ATG12 antibody (NBP3-15812) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: ATG12 Antibody (9R8W2) [NBP3-15812]

Immunohistochemistry-Paraffin: ATG12 Antibody (9R8W2) [NBP3-15812]

Immunohistochemistry-Paraffin: ATG12 Antibody (9R8W2) [NBP3-15812] - Immunohistochemistry of paraffin-embedded mouse lung using ATG12 Rabbit mAb (NBP3-15812) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation: ATG12 Antibody (9R8W2) [NBP3-15812]

Immunoprecipitation: ATG12 Antibody (9R8W2) [NBP3-15812]

Immunoprecipitation: ATG12 Antibody (9R8W2) [NBP3-15812] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug ATG12 antibody (NBP3-15812). Western blot was performed from the immunoprecipitate using ATG12 antibody (NBP3-15812) at a dilition of 1:500.

Applications for ATG12 Antibody (9R8W2)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATG12

The ATG12 gene codes a ubiquitin-like protein ATG12 that in isoform 1 is 140 amino acids long at 15 kDA and in isoform 2 is 74 amino acids long at 7 kDA. This protein is critical for autophagy (bulk protein degradation) as it is the human homolog of a yeast protein that participates in autophagy. ATG12 participates in the immune system, apoptosis and autophagy, protein stability, negative regulators of RIG-I/MDA5 signaling, and glucose/energy metabolism. It is known to interact with various genes such as HIST1H2BF, HIST1H2BE, HIST1H2BI, HIST1H2BG, and HIST1H2BC. ATG12 has been researched regarding its role in leukemia, lung cancer, myositis, retinitis, carcinoma, colorectal cancer, and myelodysplastic syndrome.

Long Name

ATG12 Autophagy Related 12 Homolog

Alternate Names

APG12, FBR93, HAPG12

Gene Symbol

ATG12

Additional ATG12 Products

Product Documents for ATG12 Antibody (9R8W2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATG12 Antibody (9R8W2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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