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ATG13 [p Ser355] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19127

Novus Biologicals, part of Bio-Techne
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NBP2-19127
NBP2-19127SS

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

ELISA, Flow Cytometry, Knockdown Validated, Western Blot

Cited:

Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ATG13 [p Ser355] Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region near Ser355 of ATG13. (Uniprot: O75143)

Reactivity Notes

A BLAST analysis was used to suggest cross reactivity with ATG13 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

Mouse reactivity reported in scientific literature (PMID: 31428575).

Modification

p Ser355

Specificity

This affinity-purified antibody directed against the phosphorylated form of human ATG13 protein isoform 1 at the [p Ser355] residue, and to isoforms 2 and 3 at the [p Ser318] residue (Uniprot: O75143). Reactivity occurs against human ATG13 [p Ser355] protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human ATG13 is minimal by ELISA and western blot.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This affinity-purified antibody directed against the phosphorylated form of human ATG13 protein isoform 1 at the [p Ser355] residue, and to isoforms 2 and 3 at the [p Ser318] residue (Uniprot: O75143). The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for ATG13 [p Ser355] Antibody

Western Blot: ATG13 [p Ser355] Antibody [NBP2-19127]

Western Blot: ATG13 [p Ser355] Antibody [NBP2-19127]

Western Blot: ATG13 [p Ser355] Antibody [NBP2-19127] - Antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13. In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blocking Buffer and probed with the primary antibody at 1ug/mL overnight at 4C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer for 45 minutes at room temperature.

Applications for ATG13 [p Ser355] Antibody

Application
Recommended Usage

ELISA

1:25000-1:175000

Flow Cytometry

1:10-1:1000

Western Blot

1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 56.6 kDa in size corresponding to human phosphorylated ATG13 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: ATG13

Alternate Names

autophagy related 13, autophagy-related protein 13, KIAA0652

Gene Symbol

ATG13

UniProt

Additional ATG13 Products

Product Documents for ATG13 [p Ser355] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATG13 [p Ser355] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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