ATG14 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36445

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ATG14 Antibodies »

Product Specifications

Immunogen

Antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG14. The immunogen is located within amino acids 270 - 320 of ATG14. Amino Acid Squence: VEEKKTTQGPDMEQSNPA

Specificity

At least three isoforms of ATG14 are known to exist; this antibody will detect all three isoforms

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATG14 Antibody - BSA Free

Western Blot: ATG14 AntibodyBSA Free [NBP2-36445]

Western Blot: ATG14 AntibodyBSA Free [NBP2-36445]

Western Blot: ATG14 Antibody - BSA Free [NBP2-36445] - ATG14 Antibody [NBP2-36445] - DHA increases the basal level of autophagy. Protein levels of ATG5, ATG12, ATG16L1, Beclin1, ATG14, p-ATG14 (Ser29), Rab7, RILP, Lamp1, CTSB, p-mTOR(Ser2448), mammalian target of rapamycin (mTOR), ULK1, p-GSK3Beta (Ser9), GSK3Beta in extracts of whole brain tissue were detected by WB. Representative Western blots are presented in panels, and quantification of the results is shown as the mean values +- SD in panels (B,D,F,H). *p less than 0.05, **p less than 0.01, and ***p less than 0.001 between two groups. One-way analysis of variance/Newman-Keuls test was performed for all WB data. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fnagi.2020.00047/full) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: ATG14 Antibody - BSA Free [NBP2-36445]

Immunocytochemistry/ Immunofluorescence: ATG14 Antibody - BSA Free [NBP2-36445]

Immunocytochemistry/Immunofluorescence: ATG14 Antibody - BSA Free [NBP2-36445] - ATG14 Antibody [NBP2-36445] - Immunofluorescence of ATG14 in human small intestine tissue with ATG14 antibody at 20 ug/mL.
Western Blot: ATG14 AntibodyBSA Free [NBP2-36445]

Western Blot: ATG14 AntibodyBSA Free [NBP2-36445]

Western Blot: ATG14 Antibody - BSA Free [NBP2-36445] - ATG14 Antibody [NBP2-36445] - Analysis of ATG14 in human small intestine tissue lysate with ATG14 antibody at (A) 1 and (B) 2 ug/mL.

Applications for ATG14 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Western Blot

1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG14

ATG14 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG14 is a subunit of a class III phosphatidylinositol (PtdIns) 3-kinase complex that targets the complex to the endoplasmic reticulum, the site of autophagosome formation.

Alternate Names

ATG14 Autophagy Related 14 Homolog, ATG14 Autophagy Related 14 Homolog (S. Cerevisiae), ATG14L, Autophagy Related 14, Autophagy-Related Protein 14-Like Protein, Barkor, Beclin 1-Associated Autophagy-Related Key Regulator, Beclin 1-interacting protein, KIAA0831

Gene Symbol

ATG14

Additional ATG14 Products

Product Documents for ATG14 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATG14 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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