ATG16L1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15504
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Bovine (94%), Mouse (93%), Porcine (93%), Rat (94%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human ATG16L1. The exact sequence is proprietary.
Reactivity Notes
Rhesus Monkey (89%).
Localization
Cytoplasm; Preautophagosomal structure membrane; Peripheral membrane protein; Cytoplasm; Preautophagosomal structure membrane
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ATG16L1 Antibody
![Western Blot: ATG16L1 Antibody [NBP2-15504]](https://resources.bio-techne.com/images/products/ATG16L1-Antibody-Western-Blot-NBP2-15504-img0001.jpg "Western Blot: ATG16L1 Antibody [NBP2-15504]")
Western Blot: ATG16L1 Antibody [NBP2-15504]
Western Blot: ATG16L1 Antibody [NBP2-15504] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.![Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-15504]](https://resources.bio-techne.com/images/products/ATG16L1-Antibody-Immunohistochemistry-Paraffin-NBP2-15504-img0002.jpg "Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-15504]")
Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-15504]
Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-15504] - Immunohistochemical analysis of paraffin-embedded U251 xenograft, using antibody at 1:500 dilution.
Western Blot: ATG16L1 Antibody [NBP2-15504] -
Western Blot: ATG16L1 Antibody [NBP2-15504] - Wild-type (WT) and ATG16L1 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG16L1 antibody [N2C1], Internal diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Applications for ATG16L1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Application Notes
ICC/IF-Assay dependent
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ATG16L1
Alternate Names
APG16 autophagy 16-like (S. cerevisiae), APG16L beta, APG16LFLJ10828, APG16-like 1, ATG16 autophagy related 16-like (S. cerevisiae), ATG16 autophagy related 16-like 1 (S. cerevisiae), ATG16A, ATG16L, autophagy-related protein 16-1, FLJ00045, FLJ10035, FLJ22677, IBD10, WD repeat domain 30, WDR30
Gene Symbol
ATG16L1
UniProt
Additional ATG16L1 Products
Product Documents for ATG16L1 Antibody
Product Specific Notices for ATG16L1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.