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ATG3/APG3 Antibody (1F7)

Novus Biologicals, part of Bio-Techne | Catalog # H00064422-M06

Novus Biologicals, part of Bio-Techne
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H00064422-M06

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1F7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ATG3 (NP_071933, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQMEYSDELEAIIEE

Reactivity Notes

Human. Other species not tested.

Specificity

ATG3 autophagy related 3 homolog (S. cerevisiae)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for ATG3/APG3 Antibody (1F7)

Western Blot: ATG3/APG3 Antibody (1F7) [H00064422-M06]

Western Blot: ATG3/APG3 Antibody (1F7) [H00064422-M06]

Western Blot: ATG3/APG3 Antibody (1F7) [H00064422-M06] - Analysis of ATG3 expression in Jurkat (Cat # L017V1).
ELISA: ATG3/APG3 Antibody (1F7) [H00064422-M06]

ELISA: ATG3/APG3 Antibody (1F7) [H00064422-M06]

ELISA: ATG3/APG3 Antibody (1F7) [H00064422-M06] - Detection limit for recombinant GST tagged ATG3 is approximately 1ng/ml as a capture antibody.

Applications for ATG3/APG3 Antibody (1F7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATG3

Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM]

Long Name

Autophagy Related 3 Homolog

Alternate Names

APG3L, Apg3p, PC3-96

Entrez Gene IDs

64422 (Human)

Gene Symbol

ATG3

OMIM

609606 (Human)

UniProt

Additional ATG3 Products

Product Documents for ATG3/APG3 Antibody (1F7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATG3/APG3 Antibody (1F7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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