ATG3/APG3 Antibody (5D2W0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15800

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15800-100ul
NBP3-15800-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5D2W0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATG3 Antibodies »

Product Summary for ATG3/APG3 Antibody (5D2W0)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATG3/APG3 (Q9NT62). MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQMEYSDELEAIIEE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATG3/APG3 Antibody (5D2W0)

Western Blot: ATG3/APG3 Antibody (5D2W0) [NBP3-15800]

Western Blot: ATG3/APG3 Antibody (5D2W0) [NBP3-15800]

Western Blot: ATG3/APG3 Antibody (5D2W0) [NBP3-15800] - Western blot analysis of extracts of various cell lines, using ATG3/APG3 antibody (NBP3-15800) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.
Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (5D2W0) [NBP3-15800]

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (5D2W0) [NBP3-15800]

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (5D2W0) [NBP3-15800] - Immunohistochemistry of paraffin-embedded mouse lung using ATG3/APG3 antibody (NBP3-15800) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (5D2W0) [NBP3-15800]

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (5D2W0) [NBP3-15800]

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (5D2W0) [NBP3-15800] - Immunohistochemistry of paraffin-embedded human lung cancer using ATG3/APG3 antibody (NBP3-15800) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for ATG3/APG3 Antibody (5D2W0)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATG3

FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoformsproduced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. SIMILARITY: Belongs to the ATG3 family.

Long Name

Autophagy Related 3 Homolog

Alternate Names

APG3L, Apg3p, PC3-96

Gene Symbol

ATG3

Additional ATG3 Products

Product Documents for ATG3/APG3 Antibody (5D2W0)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATG3/APG3 Antibody (5D2W0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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