ATG3/APG3 Antibody (OTI3H2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01083

Novus Biologicals, part of Bio-Techne
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NBP2-01083
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Conjugate
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Alexa Fluor 350
NBP2-72140AF350
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Alexa Fluor 405
NBP2-72140AF405
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Alexa Fluor 488
NBP2-72140AF488
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Alexa Fluor 532
NBP2-72140AF532
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Alexa Fluor 594
NBP2-72140AF594
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Alexa Fluor 647
NBP2-72140AF647
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Alexa Fluor 700
NBP2-72140AF700
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Alexa Fluor 750
NBP2-72140AF750
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Allophycocyanin
NBP2-72140APC
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Allophycocyanin/Cy7
NBP2-72140APCCY7
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Biotin
NBP2-72140B
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CoraFluor 1
NBP2-72140CL1
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DyLight 350
NBP2-72140UV
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DyLight 405
NBP2-72140V
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DyLight 488
NBP2-72140G
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DyLight 550
NBP2-72140R
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DyLight 594
NBP2-72140DL594
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DyLight 650
NBP2-72140C
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DyLight 680
NBP2-72140FR
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DyLight 755
NBP2-72140IR
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FITC
NBP2-72140F
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HRP
NBP2-72140H
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Janelia Fluor 525
NBP2-72140JF525
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Janelia Fluor 549
NBP2-72140JF549
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Janelia Fluor 585
NBP2-72140JF585
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Janelia Fluor 635
NBP2-72140JF635
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Janelia Fluor 646
NBP2-72140JF646
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Janelia Fluor 669
NBP2-72140JF669
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mFluor Violet 450 SE
NBP2-72140MFV450
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mFluor Violet 500 SE
NBP2-72140MFV500
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mFluor Violet 610 SE
NBP2-72140MFV610
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PE
NBP2-72140PE
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PE/Atto594
NBP2-72140PEATT594
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PE/Cy5.5
NBP2-72140PECY55
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PE/Cy7
NBP2-72140PECY7
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PerCP
NBP2-72140PCP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3H2

Concentration

1 mg/ml

View all ATG3 Antibodies »

Product Summary for ATG3/APG3 Antibody (OTI3H2)

Immunogen

Full length human recombinant protein of human ATG3(NP_071933) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

35.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATG3/APG3 Antibody (OTI3H2)

Western Blot: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083]

Western Blot: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083]

Western Blot: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Apg3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083]

Immunocytochemistry/ Immunofluorescence: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083]

Immunocytochemistry/Immunofluorescence: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Apg3.
Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083]

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083]

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (OTI3H2) [NBP2-01083] - Staining of paraffin-embedded Human tonsil using anti-Apg3 mouse monoclonal antibody.
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Applications for ATG3/APG3 Antibody (OTI3H2)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATG3

Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM].

Long Name

Autophagy Related 3 Homolog

Alternate Names

APG3L, Apg3p, PC3-96

Entrez Gene IDs

64422 (Human)

Gene Symbol

ATG3

Additional ATG3 Products

Product Documents for ATG3/APG3 Antibody (OTI3H2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATG3/APG3 Antibody (OTI3H2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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