ATG4B Antibody (9H6M6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16538

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16538-100ul
NBP3-16538-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9H6M6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATG4B Antibodies »

Product Summary for ATG4B Antibody (9H6M6)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ATG4B (Q9Y4P1). DRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATG4B Antibody (9H6M6)

Western Blot: ATG4B Antibody (9H6M6) [NBP3-16538]

Western Blot: ATG4B Antibody (9H6M6) [NBP3-16538]

Western Blot: ATG4B Antibody (9H6M6) [NBP3-16538] - Western blot analysis of extracts of various cell lines, using ATG4B Rabbit mAb (NBP3-16538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538]

Immunocytochemistry/ Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538]

Immunocytochemistry/Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538] - Immunofluorescence analysis of NIH-3T3 cells using ATG4B Rabbit mAb (NBP3-16538) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for ATG4B Antibody (9H6M6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATG4B

FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 5 named isoforms produced by alternative splicing.

Long Name

ATG4 Autophagy Related 4 Homolog B

Alternate Names

Apg4B, AUTL1

Gene Symbol

ATG4B

Additional ATG4B Products

Product Documents for ATG4B Antibody (9H6M6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATG4B Antibody (9H6M6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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