ATG4B Antibody (JE59-97)

Western Blot: ATG4B Antibody (JE59-97) [NBP3-32057]

Western Blot: ATG4B Antibody (JE59-97) [NBP3-32057] - Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (NBP3-32057) at 1/1,000 dilution.

Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: PC-12 cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 44 kDa
Observed band size: 44 kDa

Exposure time: 1 minute 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32057) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.