ATG4D Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33488

Novus Biologicals, part of Bio-Techne
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NBP1-33488
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%), Porcine (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATG4D Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG4D. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATG4D Antibody

Immunocytochemistry/ Immunofluorescence: ATG4D Antibody [NBP1-33488]

Immunocytochemistry/ Immunofluorescence: ATG4D Antibody [NBP1-33488]

Immunocytochemistry/Immunofluorescence: ATG4D Antibody [NBP1-33488] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG4D protein stained by ATG4D antibody [N3C3] diluted at 1:200.Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488]

Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488]

Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488] - Paraffin-embedded rat muscle. ATG4D antibody [N3C3] diluted at 1:500.
Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488]

Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488]

Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488] - Paraffin-embedded SAS xenograft, using antibody at 1:100 dilution.
View 1 more images

Applications for ATG4D Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATG4D

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.; FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.; ENZYME REGULATION: Inhibited by N-ethylmaleimide; SUBCELLULAR LOCATION: Cytoplasm; TISSUE SPECIFICITY: Mainly expressed in skeletal muscle and, to a lower extent, in testis.;

Alternate Names

APG4 autophagy 4 homolog D, APG4 autophagy 4 homolog D (S. cerevisiae), APG4D, APG4-D, ATG4 autophagy related 4 homolog D (S. cerevisiae), AUTL4, AUT-like 4 cysteine endopeptidase, AUT-like 4, cysteine endopeptidase, AUT-like 4, cysteine endopeptidase (S. cerevisiae), Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, cysteine protease ATG4D, cysteine protease involved in autophagy, EC 3.4.22, EC 3.4.22.-

Entrez Gene IDs

84971 (Human); 235040 (Mouse); 686505 (Rat)

Gene Symbol

ATG4D

UniProt

Q86TL0 (Human)

Additional ATG4D Products

Product Documents for ATG4D Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATG4D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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