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ATIC Antibody (OTI1D2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70220

Novus Biologicals, part of Bio-Techne
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NBP2-70220
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human ATIC(NP_004035) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

64.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATIC Antibody (OTI1D2) - Azide and BSA Free

Western Blot: ATIC Antibody (OTI1D2)Azide and BSA Free [NBP2-70220]

Western Blot: ATIC Antibody (OTI1D2)Azide and BSA Free [NBP2-70220]

Western Blot: ATIC Antibody (OTI1D2) - Azide and BSA Free [NBP2-70220] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATIC (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATIC.
Flow Cytometry: ATIC Antibody (OTI1D2) - Azide and BSA Free [NBP2-70220]

Flow Cytometry: ATIC Antibody (OTI1D2) - Azide and BSA Free [NBP2-70220]

Flow Cytometry: ATIC Antibody (OTI1D2) - Azide and BSA Free [NBP2-70220] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-ATIC antibody, and then analyzed by flow cytometry.
Western Blot: ATIC Antibody (OTI1D2)Azide and BSA Free [NBP2-70220]

Western Blot: ATIC Antibody (OTI1D2)Azide and BSA Free [NBP2-70220]

Western Blot: ATIC Antibody (OTI1D2) - Azide and BSA Free [NBP2-70220] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ATIC monoclonal antibody.

Applications for ATIC Antibody (OTI1D2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATIC

ATIC is a 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase which helps catalyze the penultimate and final steps of the de novo purine biosynthetic pathway. The human ATIC cDNA encodes a deduced 591 amino acid protein that is 81% identical to the chicken sequence.

Alternate Names

5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMPcyclohydrolase, 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotidetransformylase/inosinicase, AICAR, AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme, AICARFT, AICARFT/IMPCHASE, bifunctional purine biosynthesis protein PURH, IMPCHASE, phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, PURHFLJ93545

Gene Symbol

ATIC

Additional ATIC Products

Product Documents for ATIC Antibody (OTI1D2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATIC Antibody (OTI1D2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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