ATIC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89192

Novus Biologicals, part of Bio-Techne
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NBP1-89192
NBP1-89192-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATIC Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ANYWWLRHHPQVLSMKFKTGVKRAEISNAIDQYVTGTIGEDEDLIKWKALFEEVPELLTEAEKKEWVEKLTEVSISSDAFFPFRDNVDRAKRSGVAYIAAPSGSAADKVVIEACDELGIILAH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATIC Antibody

Western Blot: ATIC Antibody [NBP1-89192]

Western Blot: ATIC Antibody [NBP1-89192]

Western Blot: ATIC Antibody [NBP1-89192] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATIC antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: ATIC Antibody [NBP1-89192]

Immunocytochemistry/ Immunofluorescence: ATIC Antibody [NBP1-89192]

Immunocytochemistry/Immunofluorescence: ATIC Antibody [NBP1-89192] - Staining of human cell line A-431 shows positivity in plasma membrane and cytoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ATIC Antibody [NBP1-89192]

Immunohistochemistry-Paraffin: ATIC Antibody [NBP1-89192]

Immunohistochemistry-Paraffin: ATIC Antibody [NBP1-89192] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
View 1 more images

Applications for ATIC Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATIC

ATIC is a 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase which helps catalyze the penultimate and final steps of the de novo purine biosynthetic pathway. The human ATIC cDNA encodes a deduced 591 amino acid protein that is 81% identical to the chicken sequence.

Alternate Names

5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMPcyclohydrolase, 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotidetransformylase/inosinicase, AICAR, AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme, AICARFT, AICARFT/IMPCHASE, bifunctional purine biosynthesis protein PURH, IMPCHASE, phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, PURHFLJ93545

Gene Symbol

ATIC

Additional ATIC Products

Product Documents for ATIC Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATIC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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