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ATP5O Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92921

Novus Biologicals, part of Bio-Techne
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NBP2-92921-0.02ml
NBP2-92921-0.1ml

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 24-213 of human ATP5O (NP_001688.1). FAKLVRPPVQVYGIEGRYATALYSAASKQNKLEQVEKELLRVAQILKEPKVAASVLNPYVKRSIKVKSLNDITAKERFSPLTTNLINLLAENGRLSNTQGVVSAFSTMMSVHRGEVPCTVTSASPLEEATLSELKTVLKSFLSQGQVLKLEAKTDPSILGGMIVRIGEKYVDMSVKTKIQKLGRAMREIV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATP5O Antibody - BSA Free

Western Blot: ATP5O AntibodyBSA Free [NBP2-92921]

Western Blot: ATP5O AntibodyBSA Free [NBP2-92921]

Western Blot: ATP5O Antibody [NBP2-92921] - Analysis of extracts of various cell lines, using ATP5O at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: ATP5O Antibody - BSA Free [NBP2-92921]

Immunocytochemistry/ Immunofluorescence: ATP5O Antibody - BSA Free [NBP2-92921]

Immunocytochemistry/Immunofluorescence: ATP5O Antibody [NBP2-92921] - Analysis of U2OS cells using ATP5O at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: ATP5O Antibody - BSA Free [NBP2-92921]

Immunohistochemistry-Paraffin: ATP5O Antibody - BSA Free [NBP2-92921]

Immunohistochemistry-Paraffin: ATP5O Antibody [NBP2-92921] - Human thyroid cancer using ATP5O Rabbit pAb at dilution of 1:100 (40x lens).

Applications for ATP5O Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATP5O

ATP5O is a 213 amino acid F-type ATPase protein that is involved in producing ATP from ADP in the mitochondrial membrane when a proton gradient has been established across the membrane through the movement of charges across an electron transport complex in the respiratory chain. Current research is being performed on several diseases and disorders including graves disease, hashimotos thyroiditis, hypothyroidism, autoimmunity, goiter, diabetes mellitus, Huntington's disease, stomach cancer, osteoporosis, Parkinsons, twinning, osteosarcoma, hepatitis, and malaria. This protein has also been shown to have interactions with ATP5A1, ATP5B, ATP5D, IKBKG, and MPG in pathways such as the oxidative phosphorylation, metabolic, citric acid cycle (TCA), and Alzheimer's pathways.

Alternate Names

ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit, human ATP synthase OSCP subunit, oligomycin sensitivity conferring protein10ATPOATP synthase subunit O, mitochondrial, Oligomycin sensitivity conferral protein, oligomycin sensitivity conferring protein, OSCPhuman ATP synthase OSCP subunit

Gene Symbol

ATP5PO

Additional ATP5O Products

Product Documents for ATP5O Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATP5O Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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