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ATP6AP1 Antibody (3A2)

Novus Biologicals, part of Bio-Techne | Catalog # H00000537-M01

Novus Biologicals, part of Bio-Techne
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H00000537-M01

Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3A2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ATP6AP1 (NP_001174, 51 a.a. ~ 150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SSDRDLWAPAADTHEGHITSDLQLSTYLDPALELGPRNVLLFLQDKLSIEDFTAYGGVFGNKQDSAFSNLENALDLAPSSLVLPAVDWYAVSTLTTYLQE

Specificity

ATP6AP1 - ATPase, H+ transporting, lysosomal accessory protein 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ATP6AP1 Antibody (3A2)

Western Blot: ATP6AP1 Antibody (3A2) [H00000537-M01]

Western Blot: ATP6AP1 Antibody (3A2) [H00000537-M01]

Western Blot: ATP6AP1 Antibody (3A2) [H00000537-M01] - Analysis of ATP6AP1 expression in transfected 293T cell line by ATP6AP1 monoclonal antibody (M01), clone 3A2.Lane 1: ATP6AP1 transfected lysate(52 KDa).Lane 2: Non-transfected lysate.
Western Blot: ATP6AP1 Antibody (3A2) [H00000537-M01]

Western Blot: ATP6AP1 Antibody (3A2) [H00000537-M01]

Western Blot: ATP6AP1 Antibody (3A2) [H00000537-M01] - ATP6AP1 monoclonal antibody (M01), clone 3A2 Analysis of ATP6AP1 expression in HeLa.
Western Blot: ATP6AP1 Antibody (3A2) [H00000537-M01]

Western Blot: ATP6AP1 Antibody (3A2) [H00000537-M01]

Western Blot: ATP6AP1 Antibody (3A2) [H00000537-M01] - ATP6AP1 monoclonal antibody (M01), clone 3A2. Analysis of ATP6AP1 expression in PC-12.

Applications for ATP6AP1 Antibody (3A2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATP6AP1

This gene encodes a component of a multisubunit enzyme (1 mDa MW) that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene is approximately 45 kD and may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules.

Alternate Names

16A, Ac45, ATP6IP1V-ATPase subunit S1, ATP6S1ORF, ATPase, H+ transporting, lysosomal (vacuolar proton pump), subunit 1, ATPase, H+ transporting, lysosomal accessory protein 1, H+ transporting, lysosomal interacting protein 1, H-ATPase subunit, MGC129781, Protein XAP-3, Vacuolar proton pump subunit S1, VATPS1V-ATPase Ac45 subunit, V-type proton ATPase subunit S1, XAP-3, XAP3V-ATPase S1 accessory protein

Entrez Gene IDs

537 (Human)

Gene Symbol

ATP6AP1

OMIM

300197 (Human)

Additional ATP6AP1 Products

Product Documents for ATP6AP1 Antibody (3A2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATP6AP1 Antibody (3A2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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