ATP6V1A Antibody (4F5)

Novus Biologicals, part of Bio-Techne | Catalog # H00000523-M02

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 4F5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

ATP6V1A (NP_001681, 508 a.a. ~ 617 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMIAFYDMARRAVETTAQSDNKITWSIIREHMGDILYKLSSMKFKDPLKDGEAKIKSDYAQLLEDMQNAFRSLED

Specificity

Reacts with ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ATP6V1A Antibody (4F5)

Western Blot: ATP6V1A Antibody (4F5) [H00000523-M02]

Western Blot: ATP6V1A Antibody (4F5) [H00000523-M02] - Analysis of ATP6V1A expression in transfected 293T cell line by ATP6V1A monoclonal antibody (M02), clone 4F5.Lane 1: ATP6V1A transfected lysate (Predicted MW: 68.3 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/ Immunofluorescence: ATP6V1A Antibody (4F5) [H00000523-M02]

Immunocytochemistry/Immunofluorescence: ATP6V1A Antibody (4F5) [H00000523-M02] - Analysis of monoclonal antibody to ATP6V1A on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: ATP6V1A Antibody (4F5) [H00000523-M02]

Immunohistochemistry-Paraffin: ATP6V1A Antibody (4F5) [H00000523-M02] - Analysis of monoclonal antibody to ATP6V1A on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.

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Applications for ATP6V1A Antibody (4F5)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

This antibody is reactive against recombinant protein in WB and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATP6V1A

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq]

Long Name

V-type proton ATPase catalytic subunit A

Alternate Names

ATP6A1, ATP6V1A1, EC 7.1.2.2, V-ATPase subunit A, VPP2

Entrez Gene IDs

523 (Human)

Gene Symbol

ATP6V1A

UniProt

NP_001681 (Human)

Additional ATP6V1A Products

Product Documents for ATP6V1A Antibody (4F5)

Product Datasheets

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COA

SDS

Product Specific Notices for ATP6V1A Antibody (4F5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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